47 replies to this topic

[Joelynn]

Greetings all.  

On behalf of Dr. Hudson, he is picking up the mitochondrial DNA study again (Please see link to research page of his WS).  He would greatly appreciate blood samples from the Abeyyan, Hadban, Dahman, Seglawi and Rodania lines (SEE BELOW).

Copy of his email message:

Good morning Joelynn

I am beginning to collect blood samples and I appreciate your offer. Please review the attached document and tell me which of the 10 female  lines you have female  descendents from.  The collection process requires 1cc of blood, dripped onto a dna card, then mailed to me.  More on that later.

Thanks

Dr. Hudson

(Will post the atached doc when I can find it again!  LOL.  However, it is very similar to his research page).
He also stated that the Abeyyan is greatly needed.  He needs blood samples of mares only.  

Dr. Hudson can be reached at :

whudson@whudsoncardiology.com

WS:  http://zandaiarabians.com/index.html

C of Research page to his WS:


Zandai Arabians has been involved in genetic research with respect to questions of Egyptian Arabian horse purity. Beginning with the landmark article by Michael Boling in 1998 in Arabian Visions, the technique of mitochondrial DNA analysis was applied to the problem of "root mare confusion." This controversy arose in regard to the identity of the female lines of Maaroufa and Ghazieh I. Historically, Maaroufa has been considered a Koheilan Jellabi and Ghazieh I has been considered a Saqlawi Jedrania. However, conflicting historical evidence has suggested and the Boling research has confirmed that these two mares are, in fact, female descendents of a common female ancestress. This means that Maaroufa was not a Koheilan Jellabi but, in fact, a Saqlawi Jedrania.

We have reproduced these findings at Zandai Arabians with mitochondrial DNA analysis of our own horses. A pilot study was done in 2005 to determine the mitochondrial DNA sequence of five major existing EAO female lines, namely Rodania {Koheilan Rodania}, Venus (Hadban Enzahi}, Roga El Beida {Saqlawi Jedrania}, Bint El Bahrein {Dahma Shahwania}, El Shebaa {Abbeyan Om Jurays} and El Dahma {Dahma Shahwania}. The results of the 2005 study indicate that the lines of El Dahma, El Shebaa and Bint El Bahrein are unique and distinct from all of the other EAO female lines. However, the study found that the mare lines of Rodania, Venus and Roga El Beida are identical mitochondrial DNA matches, meaning that they share a common root mare ancestress. This was a most surprising result and based on this, further research is being pursued.

Zandai Arabians is currently planning a definitive large scale survey of all extant EAO female blood lines. Anyone interested in volunteering their straight Egyptian horses for this study are encouraged to sign up by contacting me by email. The study requires one tube of blood per horse, at no cost to the owner. Tail female descendents of the above named mares are needed.



....Thank you all in advance for your participation.

[diane]

thanks, Joelynn.  I'll give it some consideration - will need to investigate logistics of it all.

Joelynn, on Apr 14 2010, 04:57 PM, said:

The results of the 2005 study indicate that the lines of El Dahma, El Shebaa and Bint El Bahrein are unique and distinct from all of the other EAO female lines. However, the study found that the mare lines of Rodania, Venus and Roga El Beida are identical mitochondrial DNA matches, meaning that they share a common root mare ancestress. This was a most surprising result and based on this, further research is being pursued.

This paragraph is indeed interesting.  Something to consider for long term programs re genetic diversity.  Though it would be interesting to know if El Dahma, El Shebaa and Bint El Bahrein are individually unique or unique as a group.  Can't seem to find more info on the website.  I'm aware of El Dahma and Bint El Bahrein/Bahreyn being Dahmah Shahwanieh but who is El Shebaa?  Is this another spelling for the 1925 foundation mare El Shahbaa (INS) AK link Ancestral Elements and/or El Shahba'a / Ash-Shahba'a (as per Manual of Straight Egyptian Arabian Horses M Murdoch/TPS?

[ponygirl]

great news!

[Joelynn]

Here we go:

Mitochondrial DNA Analysis and Genealogic Evaluation of  the Egyptian Arabian Horse




INTRODUCTION

The Royal Agricultural Society/Egyptian Agricultural Organization { RAS/ EAO} Arabian horse breeding  program { Egyptian Agricultural Organization, El-Zahraa Stud, Ahmed Esmat St., Ein Shams, Cairo, Egypt}  maintains a closed horse - breeding population consisting of purebred Arabian horses.  The importance of this program  is  based in part on the belief that the  horses used to form the  herd at its inception constitute a unique genetic pool,  it that they were considered to be genetically identical to the aboriginal horse  from the Arabian peninsula interior.  Founded in 1892, the   purpose  of the EAO  is to  preserve this authentic Arab horse in its pristine state of blood purity by breeding these  horses  in a closed genetic pool, in perpetuity.

“ The horses bred by the Royal Agricultural Society are the descendents of the authentic Arabian horses imported into Egypt from the Arabian peninsula by the late Abbas Pasha I, Viceroy of Egypt .. and has been carried on  ever since by members of his family. “
“ Breeders and lovers of the authentic Arabian horses abroad are well aware of the fact most of the famous studs of Arab horses in Europe were devastated during each of the two Great Wars and Egypt will therefore  remain an important centre for breeding and preserving the authentic Arab horse.”
                                                                                         H.E. Fouad Pasha Abaza
                                                                                          Director General
                                                                                           Royal Agricultural Society
                                                                                           EAO Stud book Vol. 1  1947    

The EAO  gathered  its foundation stock from  sources of desert-bred horses  with  known  valid pedigrees, all originating from  the bedouin tribes of Arabia. The Egyptian arab  horse ,the oriental or eastern horse,  is unique and distinct among arabs. It is characterized by its  small size, sculpted gazelle-like head, large dark eyes set low in the skull, hard bones and tendons, arched neck well set on, and a spirited and proud carriage.  Desert lore details the long and often romantic association between the authentic desert horse and the Bedouin, and the Bedouin commitment to purity of the blood is  well known . These foundation horses were considered “ asil, ”   an Arabic term which means purebred or noble.   An asil horse is one  which traces in all lines to pure bred horses from  deserta arabia and contains   no  genetic material from  horses originating outside the Arabian peninsula.  This belief in blood purity in the EAO stock , held by credible sources and supported by extensive documentary evidence at the EAO  is not uniformly accepted by Arabian horse breeders.  Considerable  mainstream  skepticism exists.   The issue remains controversial  and  is  unresolved..   Some breed  authorities have  asserted  that all desert pedigrees are of doubtful veracity since the  source data  by its very  nature  cannot be corroborated.    Others authorities , like the Pyramid Society USA and The Asil Club in Germany support the view that the Egyptian Arabian stock collected and propagated  by the EAO is a genetically pure  and unique form of the desert horse which is  free from genetic alteration or admixture.  This   controversy  is succinctly expressed by Dr. Hans Nagel  in his book Hanan {1998} regarding the question of pedigrees in theArabian horses of the EAO .        “ Thus  not only the pedigree requires a large amount of trust, even the beginning itself is a test of faith and uncritical acceptance.”  There is much truth in  this .    The Arabian horse breeder invests a great deal of time and energy in his pursuit, and pedigree analysis is  considered  essential, having long  been  regarded as the cornerstone of the breeding process.  Loss of confidence in the validity of the  pedigree in general  or the discovery of an  actual error in a long  established  pedigree  will inevitably  have   far reaching effects.

The claim can be made  that the EAO horses are   in fact the only genetically  unspoiled descendents of the authentic desert Arabian horse.    Polish Arabian horses ,     considered  by many to be “pure bred,”   contain non-arabian elements.  The Polish  foundation mares included   five  heavy European tarpan-like mares possessing  no Arabian blood at all. The Polish foundation mares Szamrajowka,  Szweykowska,  Woloszka,  Ukrainka, and  Milordka , { Bial Cerkiew and Slawuta studs, about 1810} were  all non –arabians, native to Europe,  and  make up about 15% of the ancestral elements of the present day Polish Arabian horse. These mares, supplemented with  imported desert mares were bred to Arabian stallions, and the result is now called “purebred.”
One may believe in the truthfulness of a given pedigree or not , but the fact  remains  that   until recently   there has been  no  scientific or universally agreeable form of evidence that can be applied toward a resolution to this problem. With the emergence of genomic investigational  techniques, a novel form of   objective and scientific   inquiry   can  now being applied to this  old and perplexing  problem.  Modern genetic techniques  can provide an objective   method   for determining   the genetic makeup of living horses  with the goal of clarifying family relationships.   This study focuses on  the  techniques of mitochondrial  DNA analysis which  can be applied to the question of pedigree validation and verification , providing  a   new  element  in the debate regarding the  validity of  Arabian horse pedigrees.  
Beginning in 2000 with the landmark article by Michael Boling {Animal Genetics}, the technique of mitochondrial DNA {mtDNA} analysis has been applied to the problem of  "root mare confusion."   The Boling research  was prompted by a long standing controversy  regarding the  family origins of the Egyptian female lines of Maaroufa and Ghazieh I.   According to Egyptian documents ,  Maaroufa’s   was a descendent of a Koheilan Jellabi mare.  Ghazieh I  was recorded as descending from a Saqlawi Jedrania mare .  However, conflicting historical evidence has suggested and the Boling research has confirmed that these two mares are, in fact, female descendants of a common female ancestress. This means that Maaroufa’s strain was not Koheilan Jellabi but Saqlawi Jedrania, the same female line {or strain} as Ghazieh I.  This  technique of mtDNA analysis has also been used  to  investigate other  irregularities  involving root mare confusion  in the pedigrees of both Polish Arabian horses and Thoroughbred race horses.  


We have confirmed the Boling findings at Zandai Arabians with mitochondrial DNA analysis of  Straight Egyptian horses.  Our  pilot study, done in 2005,  determined  the mitochondrial DNA sequence of five major existing EAO female lines,  Rodania { Koheilan Rodania} Venus { Hadban Enzahi} , Roga El Beida  { Saklawi Jedrania}, Bint el Bahrein  { Dahma Shahwania}, El Shebaa { Abbeyan Om Jurays}  and El Dahma {Dahma Shahwania}.  The results of the 2005 study indicate that the lines of El Dahma, El Shebaa and Bint el Bahrein are unique and distinct from  all of the  other EAO female lines.  However, the study  found that the mare lines of Rodania, Venus and Roga El Beida are identical mitochondrial DNA matches. This suggests that they share a common root mare ancestress.  This was an unexpected  finding and further research is being pursued. These results have not been published and  at  are  unverified by independent research.  


Study Design: This study is designed to apply genetic testing to the validation of the pedigrees of the  10  extant  mare  lines of  the RAS/ EAO.    Questions of “root mare confusion” will be  addressed  and possible genetic inter-relationship between the families { or “strains”} will be examined. The Egyptian mare lines to be  included in the study are:
1. El Dahma, ca. 1879, n.c., Dahma Shahwaniyah, Ali Pasha Sherif
2. Bint El Bahreyn, 1898, bay, Dahma Shahwaniyah
3. Roga El Beida { Roda } 1882 grey, Saqlawi Jedraniyah, Ali Pasha Sherif
4. Ghazieh n.d. or.ar. Saqlawi Jedraniyah, Ibn Soudan of the Ruala
5. Venus {Yunis} n.d, or.  ar., Hadban Enzahiyah
6. El Obeya Om Grees, 1925 grey, Abeyyan Om Jurays
7. Rodania ca. 1869 ch. or.ar. Kuhayla Rodan
8. El Kahila, 1925 bay  {INS} King Ibn Saoud, no strain given
9. Mabrouka  1930 bay { INS} Saqlawi, King Aziz Ibn Saoud
10. El Samraa  1924 grey { Hab el-Rih x Bint el-Sheik } { INS} Sheik Omar Abdel El Hafiz, no strain given


Methodology: Mitochondrial DNA {mtDNA}, unlike nuclear DNA, is found in the mitochondria of all mammalian cells, and is transmitted to offspring only by the mother. Thus, all  produce of a particular mare have identical mtDNA, and the female to female inheritance provides a mechanism by which the validity of documentary and historical pedigrees may be examined. Each mare line has a unique mtDNA signature or “fingerprint” which is unchanged over hundreds of generations and is transmitted  from one generation to the next without alteration.  Blood samples will be obtained from living female representatives of  the Egyptian families, and  mtDNA will be extracted and sequenced using PCR technology developed specifically for this application. A set of unique genomic signatures or  haplotypes will be revealed, and this data will be compared to the recorded pedigrees. Documentary sources for this  study are the EAO Studbook Vol. 1, 1947, and Pearson, C. The Arabian Horse Families of Egypt, Alexander Heriot, 1988.
Disclosure: This research project is funded  entirely by Zandai Arabians.  The results and opinions contained herein are solely those of Zandai Arabians.  The name “Zandai” is a trademark, protected by the USPTO.

[hansi]

Joelynn, on Apr 14 2010, 04:57 PM, said:

Greetings all.  

On behalf of Dr. Hudson, he is picking up the mitochondrial DNA study again (Please see link to research page of his WS).  He would greatly appreciate blood samples from the Abeyyan, Hadban, Dahman, Seglawi and Rodania lines (SEE BELOW).

Copy of his email message:

Good morning Joelynn

I am beginning to collect blood samples and I appreciate your offer. Please review the attached document and tell me which of the 10 female  lines you have female  descendents from.  The collection process requires 1cc of blood, dripped onto a dna card, then mailed to me.  More on that later.

Thanks

Dr. Hudson

(Will post the atached doc when I can find it again!  LOL.  However, it is very similar to his research page).
He also stated that the Abeyyan is greatly needed.  He needs blood samples of mares only.  

Dr. Hudson can be reached at :

whudson@whudsoncardiology.com

WS:  http://zandaiarabians.com/index.html

C of Research page to his WS:


Zandai Arabians has been involved in genetic research with respect to questions of Egyptian Arabian horse purity. Beginning with the landmark article by Michael Boling in 1998 in Arabian Visions, the technique of mitochondrial DNA analysis was applied to the problem of "root mare confusion." This controversy arose in regard to the identity of the female lines of Maaroufa and Ghazieh I. Historically, Maaroufa has been considered a Koheilan Jellabi and Ghazieh I has been considered a Saqlawi Jedrania. However, conflicting historical evidence has suggested and the Boling research has confirmed that these two mares are, in fact, female descendents of a common female ancestress. This means that Maaroufa was not a Koheilan Jellabi but, in fact, a Saqlawi Jedrania.

We have reproduced these findings at Zandai Arabians with mitochondrial DNA analysis of our own horses. A pilot study was done in 2005 to determine the mitochondrial DNA sequence of five major existing EAO female lines, namely Rodania {Koheilan Rodania}, Venus (Hadban Enzahi}, Roga El Beida {Saqlawi Jedrania}, Bint El Bahrein {Dahma Shahwania}, El Shebaa {Abbeyan Om Jurays} and El Dahma {Dahma Shahwania}. The results of the 2005 study indicate that the lines of El Dahma, El Shebaa and Bint El Bahrein are unique and distinct from all of the other EAO female lines. However, the study found that the mare lines of Rodania, Venus and Roga El Beida are identical mitochondrial DNA matches, meaning that they share a common root mare ancestress. This was a most surprising result and based on this, further research is being pursued.

Zandai Arabians is currently planning a definitive large scale survey of all extant EAO female blood lines. Anyone interested in volunteering their straight Egyptian horses for this study are encouraged to sign up by contacting me by email. The study requires one tube of blood per horse, at no cost to the owner. Tail female descendents of the above named mares are needed.



....Thank you all in advance for your participation.

DEar Joelyn

I gladly participate. We still have here some oldies, 30-32 years old- SEs- and others.
What do I have to do? Where do I get those viles for the blood, or will hair do?
I was told that there is a difference between testing blood and hair.

Thanks
Hansi

[NAF]

Joelynn, I have a daughter of *Bintbint El Samraa that I would be glad to give the samples of blood or hair to the study, also 2 mares that have tail female to Hind #19, 1 Rodania, 4 El Dahma,  Jan

[FOLATH]

If you'd like, I can send you some samples (hair or blood) from a BLUE STAR eligible straight Bedouin source purebred Arabian mare. She is Abbeyan Sharakiyah. All her descendants came here to the USA from horses that, for a long long time, were bred in the deserts of Saudi Arabia.

Just PM me your address and instructions for sending samples if you want her participation. 8O)

[Joelynn]

Hello all!  Is your weather as beautiful as it is here?  Certainly hope so!  

Thanks and appreciation to all of you that have responded!  Please wait patiently for me to get specific instruction from Dr. Hudson on how to go forward and will relay the info ASAP as soon as I get it.

Personally think this is just a NEAT study for many reasons.  It is exciting!  Some may think the mito factor is hogwash and some believe it.  Some want to cling to how we have done things in the past and some want to evolve as our technology evolves.  Everyone has.... and has the right to, their own opinion.  Anyrate.... to me, this study is neat and cannot wait for the results!  This is very kind of Dr. Hudson to take this on.  This is on his own time and expense and I for one greatly appreciate what he is doing for us, for our horses, for our breed and also for history sake (and future sake in a way).

Have a great day and thank you again!  J

[hansi]

Joelynn, on Apr 17 2010, 05:34 PM, said:

Hello all!  Is your weather as beautiful as it is here?  Certainly hope so!  

Thanks and appreciation to all of you that have responded!  Please wait patiently for me to get specific instruction from Dr. Hudson on how to go forward and will relay the info ASAP as soon as I get it.

Personally think this is just a NEAT study for many reasons.  It is exciting!  Some may think the mito factor is hogwash and some believe it.  Some want to cling to how we have done things in the past and some want to evolve as our technology evolves.  Everyone has.... and has the right to, their own opinion.  Anyrate.... to me, this study is neat and cannot wait for the results!  This is very kind of Dr. Hudson to take this on.  This is on his own time and expense and I for one greatly appreciate what he is doing for us, for our horses, for our breed and also for history sake (and future sake in a way).

Have a great day and thank you again!  J

Thanks Joelyn, this is so kind of Dr Hudson. It might just stop rumors, false believes or whatever. I love it.

Is it not wonderful what THIS FORUM has achieved? Covering diseases, CA, SCId, LFS etc. and so many of us working so nicely together for the sake of our beloved breed.
All this happens when we are permitted to issue our opinions, ask questions, bring problems forward, etc. I cant thank our webmaster enough for this genuine oportunity and all our member posters for participating. You all are so great. thanks so much.

Have a grand day and weekend
Hansi

[anitae]

Joelyn,
Do you know what testing protocol and which laboratory is doing the analysis?
Are they using the same D-loop hypervariable region used in Dr. Ann Bowling's original research?

Anita

[Joelynn]

anitae, on Apr 17 2010, 04:44 PM, said:

Joelyn,
Do you know what testing protocol and which laboratory is doing the analysis?
Are they using the same D-loop hypervariable region used in Dr. Ann Bowling's original research?

Anita

Hello, thank you for the questions.  It would be best for you to email Dr. Hudson directly for those answers.  If you would like to even, email me at joelynnld@yahoo.com and I can forward it to him.  Thanks and have a great day, J

[Joelynn]

Good day everyone!  Got responses from Dr. Hudson regarding questions you have had.  I will C& P his responses below.  In short, over seas, Canada and any other border issues are not a problem.  He may get samples from the EAO even!  2.) He will pay any vet fees incurred by the mare owner.  3.) He has a special kit that he will provide and will be sending via email further protocl about it tomorrow. 4.)  If interested, please email him with your contact info, address, mare (s) name and registration number (s).


Again, THANK YOU!  THank you!  Thank you! .... For your participation!!




Here are his response emails  (2 of them) :

That’s very helpful. I can use both Abbeyans. Please send me the mare’s full names and AHA numbers. There are no border issues in the study. In fact I hope to get a few samples from the EAO. This study will take about 6 months and should be published within a year after completion. The samples are collected using a kit that I will provide. I’ll send you the protocol tomorrow. My co- investigator is Dr. Myriam Belanger at the University of Georgia Genomics division. We have spent about a year developing the collection and analytic tools, all of which  have been designed specifically for this study.   This study exceeds all prior  equine mtDNA   studies in terms of accuracy and reliability. The blood donors are of course the essential link in this process, and I very much appreciate all the interest.

Dr. Hudson

William M. Hudson, M.D.,F.A.C.C., P.C.

Cardiology

1400 Northside Forsyth Drive

Suite 290

Cumming, Georgia 30041

Phone: 770- 887-0472

fax: 770- 887-1105

email:  whudson@whudsoncardiology.com



and another:


I will pay for any vet fees required.

Dr. Hudson



William M. Hudson, M.D.,F.A.C.C., P.C.

Cardiology

1400 Northside Forsyth Drive

Suite 290

Cumming, Georgia 30041

Phone: 770- 887-0472

fax: 770- 887-1105

email:  whudson@whudsoncardiology.com



From: Joelynn Lofaro Dillo [mailto:joelynnld@yahoo.com]

[ELAcrisi]

Joelynn, on Apr 19 2010, 09:35 PM, said:

Good day everyone!  Got responses from Dr. Hudson regarding questions you have had.  I will C& P his responses below.  In short, over seas, Canada and any other border issues are not a problem.  He may get samples from the EAO even!  2.) He will pay any vet fees incurred by the mare owner.  3.) He has a special kit that he will provide and will be sending via email further protocl about it tomorrow. 4.)  If interested, please email him with your contact info, address, mare (s) name and registration number (s).


Again, THANK YOU!  THank you!  Thank you! .... For your participation!!




Here are his response emails  (2 of them) :

That’s very helpful. I can use both Abbeyans. Please send me the mare’s full names and AHA numbers. There are no border issues in the study. In fact I hope to get a few samples from the EAO. This study will take about 6 months and should be published within a year after completion. The samples are collected using a kit that I will provide. I’ll send you the protocol tomorrow. My co- investigator is Dr. Myriam Belanger at the University of Georgia Genomics division. We have spent about a year developing the collection and analytic tools, all of which  have been designed specifically for this study.   This study exceeds all prior  equine mtDNA   studies in terms of accuracy and reliability. The blood donors are of course the essential link in this process, and I very much appreciate all the interest.

Dr. Hudson

William M. Hudson, M.D.,F.A.C.C., P.C.

Cardiology

1400 Northside Forsyth Drive

Suite 290

Cumming, Georgia 30041

Phone: 770- 887-0472

fax: 770- 887-1105

email:  whudson@whudsoncardiology.com



and another:


I will pay for any vet fees required.

Dr. Hudson



William M. Hudson, M.D.,F.A.C.C., P.C.

Cardiology

1400 Northside Forsyth Drive

Suite 290

Cumming, Georgia 30041

Phone: 770- 887-0472

fax: 770- 887-1105

email:  whudson@whudsoncardiology.com



From: Joelynn Lofaro Dillo [mailto:joelynnld@yahoo.com]

This is very interesting and shows results being expected by me personally - not all verbal pedigree transmition in the past tells reality.

Are there also results from mDNA tests about the f.e. polish  expected non arabian ancestors, TB,, or from spanish and russian nreedings, Hungarians Maria 30?

In case of yes where can i see the results of these studies ?

[Joelynn]

ELAcrisi, on Apr 19 2010, 06:30 PM, said:

This is very interesting and shows results being expected by me personally - not all verbal pedigree transmition in the past tells reality.

Are there also results from mDNA tests about the f.e. polish  expected non arabian ancestors, TB,, or from spanish and russian nreedings, Hungarians Maria 30?

In case of yes where can i see the results of these studies ?

Hansi, Ray, Martha, ....anyone know where to find these results, if any?  As far as Dr. Hudson's study, think I am assuming the focus is on specific SE mare line families.  But someone has got to know where to find results from the above mentioned studies.

[NAF]

Joelynn,  I have 3 mares that Dr. Hudson is interested in, so glad I can provide DNA to these mares .  Hope it can provide some answers.

[desertfire7]

Hi Joelynn,

I know he is specifically interested in the SE's but I would encourage him to include the complete AL KHAMSA group as they have intensely researched the history of the Arabian horses that they accept, which also includes most Straight Egyptian Arabian horses.

BLUE STARS / Blue List and Davenports would make (IMO) a huge contribution as they are mostly closed breeding groups.  

Gayle

[anitae]

Gayle and others,

The Institute for the Desert Arabian Horse is completing mtDNA study of all available asil dam-lines in North America that were not previously done in the Bowling study. This will include all the SE lines not previously done, all the other Al Khamsa lines, as well as samples from several lines available only in Europe or South Africa.

We are working through University of California at Davis, with Dr. Penedo, and they will use the same protocol as established for the Bowling study back in the 1990s.  

These are the lines that are being done now. Some of these will not be familiar to many of you because they are rare, non-SE lines.

Al Hamdaniah, Badria, Bedowia El Hamdani, Bint Barakat, Bint El Bahreyn, Bint Karima, Bint Shaams (to try to resolve the long-standing issue over coat color), Doga (through Barakah who went to South Africa), El Dahma (the Nadra El Kebira branch to compare with the Obeya branch already done by Bowlings), El Kahila, El Samraa, El Shahbaa, Folla/Fulla, Futna, Halwaaji, Hind, Kariban, Mabrouka, Mahraa, Nafaa, Roga El Beda (El Bataa branch to compare with Wanisa branch done by Bowlings), Samirah, Sawannah, Sindidah, and Turfa.  

We also have several other samples of horses from the Middle East (desert bred).

This is being done as part of a much larger study by the Institute to establish the current genetic diversity of the asil population.

Here are the lines done by the Bowling study, supplemented by additional testing done by UC Davis during the past two years. The initial Bowling study was done both to establish the reliability of the scientific method and to identify the mtDNA haplotype of the major lines in US breeding.  For this reason, the study at that time included Egyptian lines with the most influence, as well as non-asil lines. Here are the asil lines that have been done:

Abeyah, Basilisk, Dajania, El Dahma (Obeya branch), Ferida, Ghazieh, Hadba, Haidee, Jellabiet Feysul, Layya, Nejme, Nufoud, Queen of Sheba, Reshan, Rodania, Roga El Beda (Wanisa branch), Selma, Wadduda, Urfah, Venus, Werdi.

Anita Enander, Chair
Institute for the Desert Arabian Horse




Anita

[Ray]

Hi Anita,

I guess I've been asleep, or am just confused now.  Is the Institute study underway, or complete?  I was not aware of the study being done.  

So Dr. Hudson's study would be a private study, looking for the same data the Institute has already come up with, or is working on?  Perhaps a different protocol is being used?  

Joelynn, I have not heard a thing about the study mentioned by Christina.

[anitae]

Ray,
We began the study several years ago. We ran our first samples with UC Davis from two branches of the Venus line and reported the results on "the other forum" (before we were on this forum), on the Institute's website, and in our on-line newsletter -- pages 6-7 of the Spring/Summer 2007 issue, which is on-line at
www.desertarabian.org/AlMajlis/AlMajlis-V4N2-SpringSummer07.pdf

The study was publicized at last year's Egyptian Event also, as we were soliciting samples for a few missing lines.

The mtDNA haplotype is not a commercially available test. UC Davis is running it for the Institute by  agreement for our research study.  They hope, separately, to eventually obtain samples of all other US lines, so that the original study as designed by Dr. Ann Bowling will finally be complete. By working with UC Davis, the results carry the credibility of the University research protocol established by Dr. Bowling, and the results are also posted on a research database for international use.

No schedule on when our results will be done. Best case would be by the Egyptian Event.

Anita

[Jill Erisman]

anitae, on Apr 20 2010, 11:58 AM, said:

These are the lines that are being done now. Some of these will not be familiar to many of you because they are rare, non-SE lines.

Al Hamdaniah, Badria, Bedowia El Hamdani, Bint Barakat, Bint El Bahreyn, Bint Karima, Bint Shaams (to try to resolve the long-standing issue over coat color), Doga (through Barakah who went to South Africa), El Dahma (the Nadra El Kebira branch to compare with the Obeya branch already done by Bowlings), El Kahila, El Samraa, El Shahbaa, Folla/Fulla, Futna, Halwaaji, Hind, Kariban, Mabrouka, Mahraa, Nafaa, Roga El Beda (El Bataa branch to compare with Wanisa branch done by Bowlings), Samirah, Sawannah, Sindidah, and Turfa.

Hi Anitae,

I will be most interested to learn
of your findings on Kariban!

Jill